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Microbial characterization carried out at the end of the operation confirmed that both anodic and cathodic biofilm communities had been highly dominated by the Proteobacteria phylum (>seventy two% of 16S rRNA gene sequences), followed by Firmicutes (4-11%). On this research, a microbial group previously enriched from acid mine drainage (AMD) sediments was used as inoculum in single-chamber MFCs operated at pH 3.7.

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